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phase lock gel light tubes  (Quanta Biosciences)


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    Structured Review

    Quanta Biosciences phase lock gel light tubes
    Phase Lock Gel Light Tubes, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phase+lock+gel+light+tubes/pm37595349-73-22-27
    Average 86 stars, based on 1 article reviews
    phase lock gel light tubes - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Purification:

    Article Title: Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB
    Article Snippet: The following day samples were treated with 100 μg/mL RNAse A (Qiagen) for 1 hr at 37°C. .. DNA was purified using Phase Lock Gel Light tubes (Quantabio 5Prime) according to the manufacturer’s instructions. .. DNA was eluted in 20 uL nuclease-free TE buffer, pH8 (Qiagen).

    other:

    Article Title: Region Capture Micro-C reveals coalescence of enhancers and promoters into nested microcompartments
    Article Snippet: Ideal digestion concentrations were identified by samples digested to primarily (~80%) mononucleosomal fragments (150-200 bp), few (~15-20%) dinucleosomal fragments (250-350 bp), and a faint but visible band (<5%) of trinucleosomal fragments (400-500 bp) ( ). . Micrococcal nuclease digestion Cell membranes were solubilized to extract intact nuclei by resuspending crosslinked 5M cell pellets in Micro-C Buffer #1 (MB#1; 50 mM NaCl, 10 mM Tris-HCl pH = 7.5, 5 mM MgCl2, 1M CaCl2, 0.2% NP-40 Alternative (Millipore Sigma #492018), 1x Protease Inhibitor Cocktail (Sigma-Aldrich #5056489001)) at 1M cells per 100 μL for 20 min on ice.

    Incubation:

    Article Title: Distinct gene expression by expanded clones of quiescent memory CD4 + T cells harboring intact latent HIV-1 proviruses
    Article Snippet: Flowcytometry data was analyzed with FlowJo (version 10.8.1, BD) .. Sorted cells were incubated with Proteinase K buffer (10 mM Tris, pH 8, 0.5% SDS, 100 mM NaCl, and 1 mM EDTA) and 250 μg/mL Proteinase K (ThermoFisher, cat. AM2548) at 50°C for 6 h. An equal volume of phenol-chloroform-isoamyl (Sigma-Aldrich, cat. 77617) was added and gDNA was extracted with Phase Lock Gel light tubes (QuantaBio, cat. 10847-800). gDNA was precipitated with ethanol and GlycoBlue Coprecipitant (ThermoFisher, cat. AM9515), washed twice with 70% ethanol and resuspended in TE buffer. gDNA concentration was measured by Qubit dsDNA BR Assay Kit (ThermoFisher, cat. Q32853). ..

    Article Title: Distinct gene expression by expanded clones of quiescent memory CD4 + T cells harboring intact latent HIV-1 proviruses.
    Article Snippet: Sorts were performed on BD FACS Aria IIu and BD FACS Aria III using FACSDiva software (version 2.0.2, BD). .. Flowcytometry data was analyzed with FlowJo (version 10.8.1, BD) gDNA extraction and quantification Sorted cells were incubated with Proteinase K buffer (10 mM Tris, pH 8, 0.5% SDS, 100 mM NaCl, and 1 mM EDTA) and 250 mg/mL Proteinase K (ThermoFisher, cat. AM2548) at 50 C for 6 h. An equal volume of phenol-chloroform-isoamyl (Sigma-Aldrich, cat. 77617) was added and gDNA was extracted with Phase Lock Gel light tubes (QuantaBio, cat. 10847-800). gDNA was precipitated with ethanol and GlycoBlue Coprecipitant (ThermoFisher, cat. AM9515), washed twice with 70% ethanol and resuspended in TE buffer. gDNA concentration was measured by Qubit dsDNA BR Assay Kit (ThermoFisher, cat. Q32853). ..

    Concentration Assay:

    Article Title: Distinct gene expression by expanded clones of quiescent memory CD4 + T cells harboring intact latent HIV-1 proviruses
    Article Snippet: Flowcytometry data was analyzed with FlowJo (version 10.8.1, BD) .. Sorted cells were incubated with Proteinase K buffer (10 mM Tris, pH 8, 0.5% SDS, 100 mM NaCl, and 1 mM EDTA) and 250 μg/mL Proteinase K (ThermoFisher, cat. AM2548) at 50°C for 6 h. An equal volume of phenol-chloroform-isoamyl (Sigma-Aldrich, cat. 77617) was added and gDNA was extracted with Phase Lock Gel light tubes (QuantaBio, cat. 10847-800). gDNA was precipitated with ethanol and GlycoBlue Coprecipitant (ThermoFisher, cat. AM9515), washed twice with 70% ethanol and resuspended in TE buffer. gDNA concentration was measured by Qubit dsDNA BR Assay Kit (ThermoFisher, cat. Q32853). ..

    Article Title: Distinct gene expression by expanded clones of quiescent memory CD4 + T cells harboring intact latent HIV-1 proviruses.
    Article Snippet: Sorts were performed on BD FACS Aria IIu and BD FACS Aria III using FACSDiva software (version 2.0.2, BD). .. Flowcytometry data was analyzed with FlowJo (version 10.8.1, BD) gDNA extraction and quantification Sorted cells were incubated with Proteinase K buffer (10 mM Tris, pH 8, 0.5% SDS, 100 mM NaCl, and 1 mM EDTA) and 250 mg/mL Proteinase K (ThermoFisher, cat. AM2548) at 50 C for 6 h. An equal volume of phenol-chloroform-isoamyl (Sigma-Aldrich, cat. 77617) was added and gDNA was extracted with Phase Lock Gel light tubes (QuantaBio, cat. 10847-800). gDNA was precipitated with ethanol and GlycoBlue Coprecipitant (ThermoFisher, cat. AM9515), washed twice with 70% ethanol and resuspended in TE buffer. gDNA concentration was measured by Qubit dsDNA BR Assay Kit (ThermoFisher, cat. Q32853). ..

    Extraction:

    Article Title: Distinct gene expression by expanded clones of quiescent memory CD4 + T cells harboring intact latent HIV-1 proviruses.
    Article Snippet: Sorts were performed on BD FACS Aria IIu and BD FACS Aria III using FACSDiva software (version 2.0.2, BD). .. Flowcytometry data was analyzed with FlowJo (version 10.8.1, BD) gDNA extraction and quantification Sorted cells were incubated with Proteinase K buffer (10 mM Tris, pH 8, 0.5% SDS, 100 mM NaCl, and 1 mM EDTA) and 250 mg/mL Proteinase K (ThermoFisher, cat. AM2548) at 50 C for 6 h. An equal volume of phenol-chloroform-isoamyl (Sigma-Aldrich, cat. 77617) was added and gDNA was extracted with Phase Lock Gel light tubes (QuantaBio, cat. 10847-800). gDNA was precipitated with ethanol and GlycoBlue Coprecipitant (ThermoFisher, cat. AM9515), washed twice with 70% ethanol and resuspended in TE buffer. gDNA concentration was measured by Qubit dsDNA BR Assay Kit (ThermoFisher, cat. Q32853). ..

    Centrifugation:

    Article Title: Identification of a Master Regulator of Differentiation in Toxoplasma
    Article Snippet: To extract DNA, 300 μL buffered Phenol:Chloroform:Isoamyl solution (Invitrogen) were added and then vortexed at low speed. .. Phases were separated using Phase Lock Gel Light tubes (Quantabio) and centrifugation at 21,000 × g for 5 min, followed by the addition of 300 μL chloroform and centrifugation. ..



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